American Society of Hematology

Figure 3.

$Figure 3. Apoptotic cells act independently of PGE2 and CD51 on DC migration but require complement and CD36. DCs were exposed to apoptotic Jurkat cells 4 hours before incubation with TNF-α (250 U/mL) or PGE2 (3.5 μg/mL and 7.0 μg/mL for 1 × PGE2 and 2 × PGE2, respectively) for 2 days. (A) Migration capacities of DCs tested using the Transwell migration assay. (B) Surface expression of CCR7 examined in total DC fractions by flow cytometry. Percentages of CCR7-positive cells as defined using isotype control (dashed line) are given. (C) DCs were incubated in 10% autologous complete or heat-inactivated serum, in the presence of 10 μg/mL blocking anti-CD51 or anti-CD36 antibodies for 30 minutes before the addition of PGE2 (□) or apoptotic Jurkat cells (▦). For clarity, specific migrations, normalized with the PGE2/TNFα/complete serum condition, are depicted (mean ± SD of 3 experiments). As in Figure 2C, specific migration represents the number of DCs migrating toward CCL21 minus the number of DCs migrating toward medium.$

Apoptotic cells act independently of PGE2 and CD51 on DC migration but require complement and CD36. DCs were exposed to apoptotic Jurkat cells 4 hours before incubation with TNF-α (250 U/mL) or PGE2 (3.5 μg/mL and 7.0 μg/mL for 1 × PGE2 and 2 × PGE2, respectively) for 2 days. (A) Migration capacities of DCs tested using the Transwell migration assay. (B) Surface expression of CCR7 examined in total DC fractions by flow cytometry. Percentages of CCR7-positive cells as defined using isotype control (dashed line) are given. (C) DCs were incubated in 10% autologous complete or heat-inactivated serum, in the presence of 10 μg/mL blocking anti-CD51 or anti-CD36 antibodies for 30 minutes before the addition of PGE2 (□) or apoptotic Jurkat cells (▦). For clarity, specific migrations, normalized with the PGE2/TNFα/complete serum condition, are depicted (mean ± SD of 3 experiments). As in Figure 2C, specific migration represents the number of DCs migrating toward CCL21 minus the number of DCs migrating toward medium.

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