Hierarchical clustering using genes associated with intracellular concentrations of TGN in ALL cells of patients treated with MP + MTX. (A) Hierarchical clustering of 49 ALL patients using 75 probe sets significantly associated with TGN accumulation in ALL cells after treatment with MTX + MP. Each row represents a probe set and each column a patient. Standardized expression values are shown according to the scale. Red symbols depict patients with TGN concentrations greater than 0.55 pmol/5 × 106 cells, and green symbols depict those with concentrations less than 0.55 pmol/5 × 106 cells. Listed are the Affymetrix probe set IDs, gene names, GenBank accession numbers, and coefficients of correlation (rho). A positive rho indicates a positive correlation, and a negative rho indicates a negative correlation between gene expression and intracellular TGN concentration. (B) PCA of ALL cells with high (red) and low (green) TGN concentration based on the selected probe sets. Similarities are visualized in 3-dimensional space.
