T-cell development in the absence of γ-catenin. (A) Histograms show thymocytes derived from lethally irradiated adult CD45.1⁺ recipient mice, which were reconstituted with CD45.2⁺ fetal liver cells of the indicated γ-catenin genotype. Numbers indicate the percentage of CD45.1^- (donor-derived) cells. (B) Bar graphs represent the mean (± SD) of the absolute number of CD45.1^- total and lin^- thymocytes of 5 chimeras. Dot plots show CD45.1^- thymocytes stained with CD4 and CD8 (C) or with the lineage cocktail, CD44 and CD25 (D). Numbers indicate the percentage of cells in the respective quadrants. (E) Total thymocytes were incubated for the indicated periods of time in complete culture medium (without the addition of growth factors). After collection, the cells were stained with the lineage cocktail, including CD45.1, plus CD44 and CD25 or plus CD4 and CD8. For each time point, the presence of viable DN3, DN4, or DP cells was estimated relative to a fixed number of added microspheres. The ratio at the onset of the culture was set to 100%. The graphs show the mean percentage (± SD) of surviving cells relative to the onset of the culture (100%).