SOCS-1 deficiency in T cells causes hyperproduction of IFN-γ in response to multiple stimuli. Nylon wool-purified lymph node T cells from 4-week-old Socs1^lox/-Lck-cre mice and Socs1^lox/- nontransgenic mice as controls were cultured for 48 hours in the presence of (A) plate-bound anti-CD3, (B) IL-2 or IL-2/7/15 in combination, (C) IL-12, (D) LPS, or (E) IL-1 or IL-1 + TNF in combination. Culture supernatants were then assayed for IFN-γ by ELISA. (F) T cells were simulated for 48 hours as indicated; then culture supernatants were assayed for TNF by ELISA. UT indicates untreated. The data represent the mean ± SEM of 4 to 6 experiments. Statistical significance (Mann-Whitney test): ^*P < .05 or ^**P < .001 compared with Socs1^lox/- nontransgenic.