Phosphorylation of Akt and Forkhead transcription factors upon treatment of BMCMCs with SCF. BMCMCs were treated for the indicated time periods with SCF, and the phosphorylation of (A) Akt(Ser473) and Akt(Thr308) or of (B) FOXO1a(Ser256), FOXO3a(Ser253), and FOXO3a(Thr32) was analyzed by Western blotting. Arrow indicates band corresponding to FOX01a. Comparable results were obtained in at least 3 separate experiments.