RT-PCR and NKp80 binding experiments. (A) RT-PCR analysis of NKp46, NKp30, NKG2D, NKp80, NKG2A, and CD94 in a human, and in a pigtailed and a rhesus macaque. Lane 1: 100-bp ladder (MK); lane 2: human (H) PBMCs; lane 3: pigtailed macaque (PTM) PBMCs; lane 4: rhesus macaque (RM) PBMCs. (B) Histograms show the detection of rhesus (top row) and pigtailed (bottom row) NKp80 receptors on the cell surface of transfected COS-7 cells using anti-human NKp80 mAbs (blue line). Red shaded histograms represent the negative controls stained with the second reagent alone. The cell surface level of simian NKp80 was detected with 3 different anti-human NKp80 mAbs (Lapi, Ma152, and Cer 1) in both macaques, as mean fluorescence intensity (MF) indicates in each histogram plot.