Phenotypic analysis of NKp80 and NKG2A. (A) Representative examples of double-color flow cytometric analysis within the lymphocyte compartment of PBMCs. mAbs specific to human NKp80 (top row) and NKG2A (bottom row) along with anti-CD16 mAb in rhesus (left column) and pigtailed (right column) macaques. The percentage of NKp80^pos/CD16^pos and NKG2A^pos/CD16^pos cell subsets are highlighted in bold in the upper right quadrant of the cross bar in the upper right quadrant of each dot plot. Of note, in each rhesus and pigtailed macaque whose flow cytometric analyses were chosen as representative examples of all animals studied, the percentage of total CD3^neg/HLA-DR^neg (NK) cells shown in Figure 1 and Figure 2A exactly matched with the NKp80^pos/CD16^pos (top row), NKG2A^pos/CD16^pos (bottom row) lymphocyte subsets shown in Figure 3. (B) Graphs showing the ratios of the reactivity of anti-human NKp80 (top row) and NKG2A (bottom row) mAbs that cross-reacted with CD3^neg/HLA-DR^neg versus CD16^pos (NK) lymphocytes in rhesus (left column) and pigtailed (right column) macaques.