Phenotypic analysis of NKp80 and NKG2A. Representative examples of double-color flow cytometric analysis within the lymphocyte compartment of PBMCs. (A) Cross-reactivity of mAbs specific to human NKp80 (top row) and NKG2A (bottom row) along with anti-CD3 and anti-HLA-DR mAbs in a human donor (left column), and in rhesus (middle column) and pigtailed (right column) macaques. The cells with the brightest mean fluorescence intensity in the lower right quadrant of each rhesus and pigtailed monkey dot plot corresponded to the HLA-DR^pos (or CD20^pos) lymphocyte subset that identified the B-cell population. The absolute percentage of CD3^neg/HLA-DR^neg (NK) lymphocytes positive for the relevant receptor is highlighted in bold in the upper left quadrant of the cross bar in the upper right quadrant of each dot plot. The relative percentage of total CD3^neg/HLA-DR^neg (NK) population positive for NKp80 and NKG2A is indicated in gray in the upper left quadrant of each dot plot. (B-C) Percentage (highlighted in bold in upper right quadrant of the cross bar in the upper right quadrant of each dot plot) of FITC-labeled CD8⁺ T cells (left column) and FITC-labeled CD4⁺ T cells (right column) positive for NKp80 (B) and NKG2A (C) in humans (top row), and in pigtailed (middle row) and rhesus (bottom row) macaques.