Pharmacologic inhibition of integrin α2β1 severely reduces deposition of mouse platelets on fibrillar collagen under flow. (A) Percent surface coverage area of platelets after whole mouse blood was treated with 30 μg/mL of the blocking anti-α2 antibody Ha1/29 (□), 5 mM EDTA (▪), or control hamster IgG (HamIgG; ▦). The results shown were determined by image analysis of the phase-contrast images and are the mean ± SEM (n = 5). Statistically significant inhibition was seen at each shear rate for Ha1/29 (P < .05) and EDTA (P < .05). (B) Representative phase-contrast images captured at 400, 700, 1000, and 1300 s–1 after 4 minutes of perfusion. The lines seen in the upper left portions of some panels are visual artifact and do not arise due to abnormalities in the collagen-coated surface.
