PLC1 and derived V-8 pro-B-leukemia cells show correlation between relative sensitivity to SCH66336 and relative ATP11a levels. (A) PLC1 cells were treated with the indicated concentrations of SCH66336 for the indicated amount of time. Viability is expressed as the percentage of trypan blue-excluding cells of the total number of cells present. This experiment was performed in parallel to the one shown in Figure 1A. (B) Viability of parental PLC1 cells was compared with that of PLC1 cells that had been passaged in a nude mouse (V-8 cells); the amount of SCH66336 is as indicated. Viability was measured after 48 hours of drug treatment. (C) Real-time RT-PCR analysis of ABCA1 and ATP11a expression in PLC1 and V-8 cells. Values are relative to those of actin. Note the average values of ATP11a/actin were 0.06 for B-1, 0.03 for B-1S, 4.3 for B-1R (Figure 2C), 0.03 for PLC1, 0.16 for V-8, and 18.1 for NIH3T3 fibroblasts. Error bars indicate the standard deviation.