Expression of ATP11a mRNA in FTI-resistant lymphoblasts. (A) RT-PCR using primers for murine ATP11a (top) and ABCA1 (middle) or actin (bottom) on the mouse RNAs indicated above the panel. B-1 represents the original cells in short-term culture. (B) Northern blot analysis of ATP11a mRNA expression in pro-B-leukemia cells. The blot was hybridized to a mouse ATP11a probe and washed to a stringency of 0.01 × SSC, 65°C. The positions of 28 and 18S rRNA are as indicated. (Right) Ethidium bromide-stained gel; (left) autoradiogram. (C) Real-time RT-PCR using primers for ABCA1 (left) or ATP11a (right). Values are relative to those of actin. B-1R cells expressed 140-fold more mRNA for ATP11a and 50-fold lower levels of ABCA1 than B-1S cells. Primers used are described in “Materials and methods.” Error bars indicate standard deviation.