Megakaryocyte genes fall into several classes based on their requirement for different GATA-1 functional domains. Quantitative RT-PCR analysis of expression of 10 genes in the different rescued populations. These genes fell into 4 classes: (A) genes rescued by wild-type GATA-1 and GATA-1s but not by V205G, (B) genes rescued by GATA-1 and both GATA-1s and V205G, (C) genes rescued by GATA-1 but not GATA-1s or V205G, and (D) genes not significantly affected by wild-type or either of the GATA-1 mutants. Expression in knockdown cells infected with the MIGR1 retrovirus (black bars, far left) was set to 1, whereas the fold changes observed in the wild-type (dark gray bars), GATA-1s (white bars) and V205G (light gray bars, far right) reconstitutions are shown. Means ± SD for 3 experiments are shown.