Normal bone marrow mononuclear cells display low sensitivity to cell-death stimuli compared with blasts. Flow cytometry evaluation of apoptosis of leukemic (CD3^-/CD7⁺) or normal (CD3⁺/CD7⁺, CD3^-/CD7^-) mononuclear-cell populations (patient 8), incubated for 24 hours with 25 ng/mL rapamycin. The cells were gated on the basis of FL1 (CD7-FITC)/side scatter (SSc) or FL2(CD3-PE)/SSc parameters and the percentage of annexin-positive cells was measured.