Figure 1.
![Figure 1. Time course of changes in phosphoinositide levels during fMLP stimulation of TNF-α-primed human neutrophils. Human neutrophils were labeled with [32P]Pi in the presence of 200 U/mL TNF-α, as described in “Materials and methods.” Neutrophils were washed, warmed for 5 minutes at 37°C in the presence or absence of 0.3 μM TGX-221, and then challenged with 100 nM fMLP or its vehicle for the times indicated. Incubations were terminated, lipids were extracted, and [32P] phosphoinositides were quantified as described in “Materials and methods.” Data are mean ± SD (n = 3-5) collected from separate preparations of neutrophils (where not shown, errors lie within the symbols).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/4/10.1182_blood-2005-03-0944/7/zh80160582600001.jpeg?Expires=1724247536&Signature=UcuPbISzVHd~Q6Iz06sv0bPY-aJHwqF-gwdT3aKgvQalshyrk8-aJWDk3qAaEDJI72snszNybrQ7GTCuvLPnAuYoCXLzmmFEzFxOK8oii-NC2AQOaTdkFRTW8ublQyyrBFGe3MCNgsDnvvpyHScJ1sD9kGg5YTbRz68RGCY1eqboTbyDJeyWYtCLL65G2lmVGbQE9HgTA82f4cbDs6kWiCxHnj1JRlapYudvkmKKGS-67OguJq8fO08CUHAV0KtXVbnCIHr9sVvId4f3OGYF0sWm0O2as26ItLnciD~FvnSb37OpuVlXkm4mwPorRrckTxFb28P9jsBEhT62VPepjw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time course of changes in phosphoinositide levels during fMLP stimulation of TNF-α-primed human neutrophils. Human neutrophils were labeled with [32P]Pi in the presence of 200 U/mL TNF-α, as described in “Materials and methods.” Neutrophils were washed, warmed for 5 minutes at 37°C in the presence or absence of 0.3 μM TGX-221, and then challenged with 100 nM fMLP or its vehicle for the times indicated. Incubations were terminated, lipids were extracted, and [32P] phosphoinositides were quantified as described in “Materials and methods.” Data are mean ± SD (n = 3-5) collected from separate preparations of neutrophils (where not shown, errors lie within the symbols).
