![Figure 2. Protein expression analysis of TRAIL, Bcl-2, Bcl-XL, FLIP, and FAS. U-266-1970 cells were treated with IL-6 (20 U/mL) alone or in combination with 1000 U/mL IFN-α or 1000 U/mL IFN-γ for the indicated times. (A) Western blot analysis. The cells were lysed and 20 μg total protein per lane was separated on NuPAGE 10% Bis-Tris (Bis[2-hydroxyethyl]imino-tris[hydroxymethyl]methane) gel and Western blot analysis was performed using specific antibodies targeting Apo2L/TRAIL, Bcl-2, Bcl-XL, FLIP, and β-actin. Three experiments were performed and one representative Western blot is shown. (B) FACS analysis. FAS surface expression was determined by flow cytometry using a primary antibody against Fas and a FITC-conjugated secondary antibody. The bars represent the fold induction of mean fluorescence intensity (MFI) as compared to cells treated with IL-6 alone. Data are expressed as mean ± SD, n = 3. The asterisks indicate significant differences to the corresponding control values (P < .05).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/4/10.1182_blood-2004-04-1322/7/zh80160582700002.jpeg?Expires=1722711095&Signature=iJ60u4WFvPmNaeda~XLtgmg5PTHtu6X22bU-~Z2GJKiftnxRdAL3R2NFmF-8jURSAkbk1PO-65b2nRu0df39cXmEFW5zSRQsb2PMj6txOwu-GXgzDf2dQqkoo~JIh8ypYhjc-41BX08BVy787z16eGjWiV~yF3noSlVZ7TIGMG8S4SnkFV84DVHm6NevIertN-apkcYkN50gFdg5kYI37rIqVM4zlnRkG9444WVl6Pl2fHOaIuNk6cbPZ~xi5nhBbviVvudP4Vy8y8JEwPW8qaEbT8rNBi7uDl~r7pkzfdxYOPnuzHP8l0z6lIPXkBMBQuw8UoNEKVKKLWVis~8bjA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Protein expression analysis of TRAIL, Bcl-2, Bcl-XL, FLIP, and FAS. U-266-1970 cells were treated with IL-6 (20 U/mL) alone or in combination with 1000 U/mL IFN-α or 1000 U/mL IFN-γ for the indicated times. (A) Western blot analysis. The cells were lysed and 20 μg total protein per lane was separated on NuPAGE 10% Bis-Tris (Bis[2-hydroxyethyl]imino-tris[hydroxymethyl]methane) gel and Western blot analysis was performed using specific antibodies targeting Apo2L/TRAIL, Bcl-2, Bcl-XL, FLIP, and β-actin. Three experiments were performed and one representative Western blot is shown. (B) FACS analysis. FAS surface expression was determined by flow cytometry using a primary antibody against Fas and a FITC-conjugated secondary antibody. The bars represent the fold induction of mean fluorescence intensity (MFI) as compared to cells treated with IL-6 alone. Data are expressed as mean ± SD, n = 3. The asterisks indicate significant differences to the corresponding control values (P < .05).
