Figure 4.
![Figure 4. NK cells induce a Ca2+ flux in iDCs followed by translocation of microtubule organizing center and IL-18 at the immunologic synapse. (A) NK cells were added to Fura2-AM-loaded iDCs and Ca2+ waves were monitored. Results are expressed as [Ca2+]i and represent the mean of calcium response of 15 different cells. (B) iDCs or iDC/NK conjugates after 3 hours of culture were stained for tubulin (green) or IL-18 (red). Nuclei are stained in blue by DAPI. Arrows point to the clustering of tubulin and IL-18 at the contact site between NK cells and iDCs.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/2/10.1182_blood-2004-10-3906/6/zh80140581310004.jpeg?Expires=1724847792&Signature=0oc7ZqbvqLbs7EpqM7qqHQrrogxC~KKjgCcF~Lim7FaS4s2m4RfUfesHNUeofIySaE0ZH-JcRXP7JyljKdiW3ThkON3yJgpzwbWDEc3KBKVAdnZktyJkeFFvs6ERU4b3O1FObwkhqxMusSZd28V6zPz6xYjbFWuXi2jWcUfxEeONlifMCgrAQw95VmjZ4uYZKJC0Cd3wNTn0INgujB7R9OmCA~cSDLQP28py65HVDB3860a6aXA~hCxSL3xgjRVBvAH3Jj8SeNqVCU4Rdkpz3yWQHJIcSdQqYcQTse19Qm45-05-V6KDc2xFSOeyJRokJrK2ZxACbF6lkYsdVc2xXg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
NK cells induce a Ca2+ flux in iDCs followed by translocation of microtubule organizing center and IL-18 at the immunologic synapse. (A) NK cells were added to Fura2-AM-loaded iDCs and Ca2+ waves were monitored. Results are expressed as [Ca2+]i and represent the mean of calcium response of 15 different cells. (B) iDCs or iDC/NK conjugates after 3 hours of culture were stained for tubulin (green) or IL-18 (red). Nuclei are stained in blue by DAPI. Arrows point to the clustering of tubulin and IL-18 at the contact site between NK cells and iDCs.
