Genes down-regulated in all mutants are expressed in erythroid cells. (A) Expression profile of the erythroid gene set. As erythropoiesis is disrupted in all mutants and in scl^mo embryos, the set of globally down-regulated genes at 36 hpf was linked to the erythroid lineage. Columns represent mutants of which name, tested allele, and the respective developmental stage are given; rows represent genes. Fold changes are shown as log2 (mutant/WT, or morphant/uninjected). Blue and yellow boxes indicate decreased and increased gene expression, respectively, in mutant compared with WT (morphant compared with uninjected) embryos. The expression of genes highlighted in red is illustrated by in situ hybridization in panels B, C, and D. (B) Expression of bzrp in WT and in clo^m39 embryos at 36 hpf. Transcripts of bzrp are abundantly detected in circulating erythrocytes in WT, but only in a limited number of cells in the trunk around the posterior part of the yolk tube extension in clo^m39 (arrowhead). (C) Transcripts of Dr.164 96.1.A1 are present in cells of the ICM of WT embryos, but absent in clo^m39 (both shown at 14-somite stage). (D) At 36 hpf, Dr.164 96.1.A1 is expressed in circulating erythrocytes in WT, but not in clo^m39 embryos.