Immunohistochemical staining patterns for CK19, CK18, and AGPR in the multiple AA-treated rat liver receiving hMSC transplantation. Rats were treated with multiple AA and hMSCs were transplanted into the rat by means of direct injection into their liver. (A) Sections of injured rat liver (day 28) from a rat that did not receive a transplant were not stained at all with antibodies specific for human CK19, CK18, and AGPR. (B) Serial cryosections obtained from the samples as shown in Figure 2C were immunostained using monoclonal antibodies specific for human CK19 (i-vi), CK18 (vii-xii), and AGPR (xii-xviii). Panels Biv-vi, x-xii, xvi-xviii, and xxii-xxiv are magnified images of corresponding squared areas of panels Bi-iii, vii-ix, xiii-xv, and xix-xxi, respectively. Similar results were obtained in 2 independent experiments. Scale bars represent 20 μm. Original magnifications: × 100 (A); × 200 (Bi-iii, vii-ix, xiii-xv, and xix-xxi); and × 400 (Biv-vi, x-xii, xvi-xviii, and xxii-xxiv).