Mig alters CCR3 receptor-ligand interaction. (A) Binding of 10 nM biotinylated eotaxin-1 (b-Etx1, black line) without (A) or with 200 nM eotaxin-2 (B) to the surface of eosinophils compared with control chemokine (biotinylated JE, b-JE, filled histogram) is shown. Representative histogram from 3 experiments is shown. (C) Binding of eotaxin-1 (black line) with or without 500 nM Mig (dashed line) is shown. A representative histogram is shown (n = 3). (D) Percent eotaxin-1 binding in the presence of 20 to 2000 nM Mig compared with eotaxin-1 alone is shown. Data represent mean ± SD of mean channel fluorescence of eotaxin-1 binding in the presence of Mig compared with eotaxin-1 alone (n = 3 experiments).