SCL and Xaml mRNA levels are reduced in xNAP1L MO–injected embryos. Eggs from a single female Xenopus were injected with xNAP1L MO or control MO (12 ng) and allowed to develop to stage 26. In situ hybridization of these embryos shows normal levels of SCL mRNA in the VBIs of control MO–injected embryos (A) and greatly reduced levels in embryos injected with xNAP1L MO (B). Similarly, Xaml expression in the VBI is not affected by control MO (C) but is reduced by injection of xNAP1L MO (D). Xaml mRNA in the nasal placodes is unaffected by xNAP1L MO injection (arrows in C-D).