xNAP1L-depleted embryos are morphologically normal but lack globin expression. Eggs from a single female Xenopus were injected with xNAP1L MO or control MO (12 ng) and allowed to develop to stage 33. xNAP1L MO–injected embryos (A, right) do not differ morphologically from control MO–injected embryos (A, left). αT₄-globin mRNA was detected by in situ hybridization³⁸  in control embryos (B, ventral view) but not in xNAP1L MO–injected embryos (C, ventral view).