Expression of Mcl-1 in CLL B cells by immunoblot analysis. Representative immunoblot data showing up-regulation of Mcl-1 by NLCs or rhBAFF. CLL B cells were cultured with or without NLCs or with SDF-1α (500 ng/mL) or rhBAFF (50 ng/mL) for 24 hours. Whole cell lysates were then prepared. The protein content was normalized to 12.5 μg and analyzed by immunoblot analysis with antibodies specific for Mcl-1, Bax, Bcl-2, or β-actin using ECL-based detection.