Figure 7.
Figure 7. γδ T cells activate DCs and enhance allogeneic responses. II-/- B6 mice were depleted of γδ T cells, irradiated with 1100 cGy TBI, and injected on day -1 with 5 × 106 B6 DCs that had been cocultured either with γδ T cells or with medium. Mice underwent transplantation with 5 × 106 T cells from bm12 donors on day 0. Splenocytes and serum were harvested on day 5 after BMT (n = 4 per group), and donor CD4+ T cells were phenotyped by FACS. Data represent the mean + SE. (A) Donor CD4+ T cells. ▥ vs ▪, *P < .05. (B) TNF-α. □ vs ▪, *P < .05. ND indicates not detected.

γδ T cells activate DCs and enhance allogeneic responses. II-/- B6 mice were depleted of γδ T cells, irradiated with 1100 cGy TBI, and injected on day -1 with 5 × 106 B6 DCs that had been cocultured either with γδ T cells or with medium. Mice underwent transplantation with 5 × 106 T cells from bm12 donors on day 0. Splenocytes and serum were harvested on day 5 after BMT (n = 4 per group), and donor CD4+ T cells were phenotyped by FACS. Data represent the mean + SE. (A) Donor CD4+ T cells. ▥ vs ▪, *P < .05. (B) TNF-α. □ vs ▪, *P < .05. ND indicates not detected.

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