Enhanced expression of the FA/BRCA pathway in melphalan-resistant 8226/LR5 and U266/LR6 cells. (A) Real-time RT-PCR was performed using ABI low-density array card. Fold values were obtained by externally standardizing against identical amplifications in drug-sensitive 8226/S cells and by internally standardizing against GAPDH in each cell line. Data shown are mean value and SD. (B) FANCD2-L relative expression was elevated after melphalan treatment. Experiments were repeated 3 times. (Top) Representative blots are shown. β-Actin blot served as loading control in all blots. (Bottom) The FANCD2-L/S ratio was quantified using densitometry. The mean values and standard deviations from 3 independent experiments are shown. Student t test was used for statistical analysis. ^*P < .05.