Schematic of a self-inactivating vector carrying WT1-specific TCR α and β chain genes. The coding region of WT1-specific TCR genes was obtained from cDNA of the WT1-specific Tc clone, TAK-1, by a 5′-RACE–PCR method. Genes encoding WT1-specific TCR α and β chains were inserted into self-inactivating lentivirus vector (CS II-MCS-IRES-GFP) as described in “Materials and methods,” and CS II-β-IRES-α was constructed. Ψ indicates packaging signal; RRE, rev responsive element; cPPT, central polypurine tract; CTS, central termination sequence; EF-1α, human elongation factor 1α subunit gene promoter; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element; LTR, long terminal repeat; and IRES, internal ribosome entry site.