American Society of Hematology

Figure 6.

$Figure 6. IL-6 accelerates proliferation of uncommitted progenitors. Five thousand cells from the sorted c-kithiSca-1+GFP– fraction from wild-type, RAG1-GFP animals were cultured as in Figure 5 and total cell numbers were counted at day 2 (A). The cells were analyzed for expression of lineage markers (CD2, CD3, CD8, CD19, B220, Mac-1, Gr1) and were designated as either Lin+ or Lin–. The Lin– fraction was further analyzed for Sca-1 expression and for levels of c-Kit. The cell number after culture is shown as absolute numbers of duplicate wells (± SD) when cultured without (□) or with (▪) IL-6. The results are representative of 2 separate experiments. (B) Sorted c-kithiSca-1+GFP– fraction from wild-type, RAG1-GFP animals was cultured as in panel A for 72 hours, gated on Lin–, c-Kithi, and analyzed for levels of Sca-1 and GFP. The results are representative of 2 separate experiments.$

IL-6 accelerates proliferation of uncommitted progenitors. Five thousand cells from the sorted c-kit^hiSca-1⁺GFP^– fraction from wild-type, RAG1-GFP animals were cultured as in Figure 5 and total cell numbers were counted at day 2 (A). The cells were analyzed for expression of lineage markers (CD2, CD3, CD8, CD19, B220, Mac-1, Gr1) and were designated as either Lin⁺ or Lin^–. The Lin^– fraction was further analyzed for Sca-1 expression and for levels of c-Kit. The cell number after culture is shown as absolute numbers of duplicate wells (± SD) when cultured without (□) or with (▪) IL-6. The results are representative of 2 separate experiments. (B) Sorted c-kit^hiSca-1⁺GFP^– fraction from wild-type, RAG1-GFP animals was cultured as in panel A for 72 hours, gated on Lin^–, c-Kit^hi, and analyzed for levels of Sca-1 and GFP. The results are representative of 2 separate experiments.

This Feature Is Available To Subscribers Only