American Society of Hematology

Figure 4.

$Figure 4. Surface phenotype of single-cell cultures of uncommitted progenitors. Lin–c-kithiSca-1+GFP– fraction containing uncommitted progenitors was isolated from RAG1-GFP knock-in background, wild-type, and SHIP–/– mice. The cells were cultured in the presence of the OP9 stromal cell or with SCF, IL-7, and FL as previously described.22,28 IL-6 (2 ng/mL) was added as indicated and the cells were cultured for 10 to 14 days. The phenotype of colonies that emerged were examined by staining with anti-CD19 and anti–Mac-1 antibodies and by GFP expression. The profiles shown were used to define the phenotype of colonies summarized in Table 2.$

Surface phenotype of single-cell cultures of uncommitted progenitors. Lin^–c-kit^hiSca-1⁺GFP^– fraction containing uncommitted progenitors was isolated from RAG1-GFP knock-in background, wild-type, and SHIP^–/– mice. The cells were cultured in the presence of the OP9 stromal cell or with SCF, IL-7, and FL as previously described.^22,28 IL-6 (2 ng/mL) was added as indicated and the cells were cultured for 10 to 14 days. The phenotype of colonies that emerged were examined by staining with anti-CD19 and anti–Mac-1 antibodies and by GFP expression. The profiles shown were used to define the phenotype of colonies summarized in Table 2.

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