American Society of Hematology

Figure 1.

$Figure 1. Hematopoietic progenitor populations in wild-type and SHIP–/– mice. Bone marrow cells from wild-type (top row) and SHIP–/– (bottom row) mice, both of which were RAG1-GFP knock-in background, were depleted of Lin+ (CD2, CD3, CD8, CD19, B220, Mac-1, Gr1, TER-119) cells. The Lin– fraction was analyzed for c-Kit, Sca-1, and GFP expression. Contaminating Lin+ cells were gated out, and the Lin– population was separated into c-KithiSca-1+ (left column) and c-KitloSca-1lo (right column) for GFP analysis. The number in each panel indicates the percentage of GFP+ cells.$

Hematopoietic progenitor populations in wild-type and SHIP^–/–mice. Bone marrow cells from wild-type (top row) and SHIP^–/– (bottom row) mice, both of which were RAG1-GFP knock-in background, were depleted of Lin⁺ (CD2, CD3, CD8, CD19, B220, Mac-1, Gr1, TER-119) cells. The Lin^– fraction was analyzed for c-Kit, Sca-1, and GFP expression. Contaminating Lin⁺ cells were gated out, and the Lin^– population was separated into c-Kit^hiSca-1⁺ (left column) and c-Kit^loSca-1^lo (right column) for GFP analysis. The number in each panel indicates the percentage of GFP⁺ cells.

This Feature Is Available To Subscribers Only