American Society of Hematology

Figure 2.

$Figure 2. Arf-expressing cells have decreased CFU-S activity. (A) After 10 days of culture, BM cells from wild-type, ArfGFP/+, and ArfGFP/GFP mice were stained with Lin-PE antibody cocktail and sorted for Lin–, GFP+, and GFP– fractions. Sorted cells were transplanted into sublethally irradiated mice at different doses. Spleens were harvested 13 days later, stained with Bouin solution, and CFU-S colonies counted and photographed. Error bars indicate standard deviation. (B) Analysis of number of CFU-S colonies formed after injection of 1 × 104 sorted cells as described in panel A. Number of CFU-S colonies represent an average of 3 to 4 spleens.$

Arf-expressing cells have decreased CFU-S activity. (A) After 10 days of culture, BM cells from wild-type, Arf^GFP/+, and Arf^GFP/GFP mice were stained with Lin-PE antibody cocktail and sorted for Lin^–, GFP⁺, and GFP^– fractions. Sorted cells were transplanted into sublethally irradiated mice at different doses. Spleens were harvested 13 days later, stained with Bouin solution, and CFU-S colonies counted and photographed. Error bars indicate standard deviation. (B) Analysis of number of CFU-S colonies formed after injection of 1 × 10⁴ sorted cells as described in panel A. Number of CFU-S colonies represent an average of 3 to 4 spleens.

This Feature Is Available To Subscribers Only