Determination of the optimal peptide concentration for MLPC. The reaction is specific because it is dependent on peptide concentration analyzed by ELISPOT assays for IFN-γ (A) and granzyme B (B). Specific CD8⁺ T-cell activation evaluated by IFN-γ and granzyme B release was dependent on the R3 peptide concentration indicating peptide-specific T-cell answers. The optimal dose of 20 μg peptide per milliliter was used in consecutive assays. Error bars indicate standard deviation.