Figure 6.
Role of PKC-δ and -α in LTB4 enhancing AM killing of opsonized K pneumoniae. Rat AMs were pretreated with vehicle control, the PKC-δ inhibitor rottlerin (6 μM), or the PKC-α inhibitor RO32-0432 (1 nM) for 20 minutes before the addition of opsonized K pneumoniae. Thirty minutes after infection, drugs were added back with or without 1 nM LTB4, as described above. Microbicidal activity was assessed; data are expressed as mean ± SE. Percentage of survival of ingested bacteria of quadruplicate values from 1 of 4 representative experiments. *P < .05 compared with untreated control; ANOVA.
