Figure 4.
Figure 4. Effect of exogenous and endogenous 5-LO metabolites on H2O2 release in rat AMs. (A) 5 × 105 rat AMs were plated and incubated with indicated concentrations of LTB4, LTC4, LTD4, 5-(S) HETE, or vehicle control (—) for 60 minutes. (B) 5 × 105 rat AMs were plated and preincubated with 10 μM AA-861, 1 μM MK886, 10 μM CP105696, or 10 μM MK571 for 20 minutes and then infected with 50:1 K pneumoniae. The H2O2 concentration in medium was determined using Amplex red as a probe. Results represent the mean ± SE from 3 independent experiments performed in triplicate. *P < .05 versus control. #P < .05 versus K pneumoniae group by ANOVA.

Effect of exogenous and endogenous 5-LO metabolites on H2O2 release in rat AMs. (A) 5 × 105 rat AMs were plated and incubated with indicated concentrations of LTB4, LTC4, LTD4, 5-(S) HETE, or vehicle control (—) for 60 minutes. (B) 5 × 105 rat AMs were plated and preincubated with 10 μM AA-861, 1 μM MK886, 10 μM CP105696, or 10 μM MK571 for 20 minutes and then infected with 50:1 K pneumoniae. The H2O2 concentration in medium was determined using Amplex red as a probe. Results represent the mean ± SE from 3 independent experiments performed in triplicate. *P < .05 versus control. #P < .05 versus K pneumoniae group by ANOVA.

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