Figure 5.
Arf6 but not Rho family small G proteins controls PI5KIα activation upon CD16 stimulation. (A) Cultured human NK cells were left uninfected or infected with the indicated recombinant vaccinia viruses. Lysates from 2 × 107 unstimulated or CD16-stimulated cells (5 minutes) were precipitated with anti-PI5KIα Ab. Immunoprecipitates were assayed for kinase activity. The panel derives from the autoradiography of 2 different TLC plates of the same experiment. (B) An aliquot of cell lysate derived from the same experiment was analyzed by immunoblotting. (C) The overexpressed constructs are shown. One representative experiment of 3 performed is shown.
