American Society of Hematology

Figure 3.

$Figure 3. CD16 stimulation induces a transient PI5KIα membrane translocation. Unstimulated or CD16-stimulated cultured NK cells (1 × 108/sample) were fractionated as described in “Materials and methods.” (Top) PI5KIα immunoprecipitates were obtained from protein extract derived from membrane and soluble fraction, loaded on 8% SDS-PAGE, and analyzed by immunoblotting with anti-PI5KIα Ab. (Middle and bottom) Total cell extract derived from the samples of the same experiment was loaded on 8% SDS-PAGE and analyzed by immunoblotting. The equivalent protein amount within the lanes was checked by Ponceau S red staining. One experiment representative of 3 performed is shown.$

CD16 stimulation induces a transient PI5KIα membrane translocation. Unstimulated or CD16-stimulated cultured NK cells (1 × 10⁸/sample) were fractionated as described in “Materials and methods.” (Top) PI5KIα immunoprecipitates were obtained from protein extract derived from membrane and soluble fraction, loaded on 8% SDS-PAGE, and analyzed by immunoblotting with anti-PI5KIα Ab. (Middle and bottom) Total cell extract derived from the samples of the same experiment was loaded on 8% SDS-PAGE and analyzed by immunoblotting. The equivalent protein amount within the lanes was checked by Ponceau S red staining. One experiment representative of 3 performed is shown.

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