Figure 1.
CD16 stimulation induces PI3K-dependent Arf6 activation in human NK cells. (A) Cell lysates derived from unstimulated, anti–CD16- or anti–MHCI-stimulated cultured human NK cells (2.5 × 107/sample) were incubated with glutathione Sepharose beads conjugated with GST-GGA3 fusion protein or GST alone. The precipitated protein (top) or an aliquot of the samples derived from the same experiment (bottom) was separated by 12% SDS-PAGE. Western blot analysis with anti-Arf6 mAb was performed. (B) NK cells were pretreated with LY294002 (50 μM) or control medium (vehicle) and left unstimulated or stimulated with anti-CD16 mAb for 10 minutes. Cell lysates were precipitated and analyzed as above. One experiment representative of 4 performed is shown.
