Bone marrow stem-cells from GATA-2^+/+ and GATA-2^+/– animals display similar self-renewal potential. Two million bone marrow nucleated cells from male mice of each genotype were injected into female wild-type recipient mice that had been subjected to lethal whole body irradiation. Recipient mice were monitored daily for survival for 2 months. Y-chromosome PCR was used to assess donor contribution in transplantations (data not shown). Irradiated mice injected with PBS alone died within 10 to 14 days of irradiation (data not shown). After 2 months, marrow suspensions were prepared and 2 million nucleated cells were injected into new female irradiated animals. Another 3 transplantations were performed sequentially at 2-month intervals. For each serial transplantation, 10 recipients were injected with each genotypic group. The cumulative survival from the tertiary transplantation onwards is displayed (A). Limiting dilution long-term culture was performed on donor cells prior to transplantation (BMT 0) and after each cycle of transplantation (B) (n = 3 for each transplant, P > .05 for each transplantation cycle). The BMT 0 sample was prepared by pooling cells from the 3 donor mice that initially underwent transplantation. Error bars depict the standard error of the mean. Statistical analysis was performed using the Student t test.