Reduced expression of GATA-2 within GATA-2^+/– bone marrow. RNA prepared from the total bone marrow (A) and Lin^–c-kit⁺Sca-1⁺ cells (B) of each genotype was subjected to reverse transcriptase reaction and real-time PCR for GATA-2 and normalized to GAPDH. For each Lin^–c-kit⁺Sca-1⁺ experiment, marrow cells from 2 to 3 animals of each genotype were pooled for cell sorting. Triplicates were used for each PCR reaction and the figures represent n = 2 experiments plus or minus SEM.