GR binds to 2 nGREs in vivo. (A) DC27.1 T cells were either untreated (-) or treated (+) with 10^-7 M Dex. Binding of GR to the -700, -500, and +160 CD95L promoter regions was assessed by chromatin immunoprecipitation (ChIP) assay. A known functional GRE-containing region of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) was used as a positive control for GR binding. As a negative control, the GR occupancy at the non-GRE-containing fragment of CD95L intron region approximately 7.5 kb downstream of the CD95L promoter was monitored. (B) Quantitative real-time PCR of DNA elements obtained from a ChIP experiment. Shown is percent of input precipitated of 1 of 3 experiments with similar results. Error bars represent mean ± SEM.