GC treatment does not affect CD95 signaling but regulates CD95L expression. (A) Primary murine day-5 T cells (left) and human day-6 T cells (right) were treated for 18 hours with the indicated concentrations of LZ-CD95L in the presence or absence of the indicated concentrations of Dex. Apoptosis was determined by propidium iodide-FACS analysis. (B) Murine day-5 T cells (left) and DC27.1 T cells (right) were (re-) stimulated by anti-CD3 antibody in the presence or absence of Dex (10^-8, 10^-7, and 10^-6 M) for 8 hours. CD95L protein expression on the cell surface was assayed by FACS analysis. Of 5 experiments, 1 representative is shown. Inset graphs show histograms for CD95L staining.