GCs inhibit AICD in primary peripheral T lymphocytes. (A) DC27.1 T cells were stimulated by anti-CD3 antibody for 18 hours in the presence or absence of the indicated concentrations of Dex or of the antagonistic anti-CD95L antibody MFL3 (10 μg/mL). (B) Same as in panel A, but using primary murine T cells 5 days after initial stimulation with ConA (day-5 T cells). (C) Same as in panel A, but using primary human T cells 6 days after initial stimulation with PHA (day-6 T cells), 10^-7 M Dex, and the antagonistic anti-CD95L antibody NOK1 (10 μg/mL). Error bars represent mean ± SEM. (D-E) Primary murine (D) and human (E) T cells were restimulated by anti-CD3 antibody for 18 hours in the presence or absence of 10^-7 M Dex at the indicated time point after initial T-cell stimulation. Apoptosis was determined by FACS analysis (FSC/SSC method for A-C and the Nicoletti et al30 method for D-E). Results are representative of at least 3 independent experiments.