Thrombocyte clustering. (A) Thrombocyte clustering in zebrafish blood and initial recruitment of young thrombocytes at the site of injury. Top to bottom, the panels show 4 independent thrombocyte clusters. Left to right, the panels represent a bright field image, a fluorescent image when using B-2 filter cube (shows DiI-labeled thrombocytes and mepacrine-labeled thrombocytes as green or orange), and a fluorescent image when using G-2A filter (DiI-labeled thrombocytes). (B, top) Kinetics of thrombocyte clustering. Panels of fluorescent images from left to right show the representative sizes of thrombocyte aggregates formed after ADP activation. Individual thrombocytes (orange and green), small orange aggregates, large green aggregates, and composite aggregates are shown at 0, 3, 5, and 10 minutes, respectively. At 5 and 10 minutes, small orange and large green aggregates are also seen but are not shown here. (Bottom) The graph shows the number of different types of aggregates obtained over a 10-minute period.