Evaluation of antigen-specific immune response in MGL1-deficient mice. (A) Mice (□, wild type; ▪, MGL1-deficient) were intradermally immunized with ABA-AcBSA and the spleen was taken 3 weeks after immunization. Splenocyte proliferation in response to ABA-AcBSA (ordinate) expressed as the ratio of the [³H]-thymidine incorporation count per minute (cpm) given by cells exposed to ABA-AcBSA to the cpm given by unexposed cells. Each value represents mean plus or minus SD(n = 6). (B-D) In other experiments, wild-type mice (B; open symbols) and MGL1-deficient mice (C; closed symbols) were subcutaneously immunized with ABA-AcBSA on day –10, and were subsequently challenged by injecting the antigen into the dorsal air pouch on days 0 and 5. Sera were collected on days –10, 4, 11, and 18. Each serum sample was prepared from 5 mice. The binding of IgG to immobilized ABA-AcBSA (ordinate) was measured for serum samples collected on days –10 (no symbol), 4 (circles), 11 (triangles), and 18 (squares) and plotted to the serum dilution (abscissa) as determined by means of an ELISA.