Identification of TC groups in MM by RNA expression profiling. The samples are 6 peripheral blood-generated plasmablasts and 1 reactive plasmacytosis (PB) (5), 14 bone marrow PCs (PC) from healthy volunteers, samples from newly diagnosed (231) or relapsed (30) MM, and 32 human myeloma cell lines (HMCL). In addition they are all samples from patients with newly diagnosed (231) or relapsed MM (30). Among these 261 samples there are 42 with elevated FGFR3 and/or MMSET (4p16), 19 with elevated MAF or ITGB7 and CX3CR1 as surrogates for MAFB (maf), 7 with elevated CCND3 (6p21), 40 with high CCND1 (11q13), 81 with lower levels of CCND1 (D1), 21 with lower levels of CCND1 and elevated CCND2 (D1+D2), 45 remaining patients with elevated CCND2 (D2), and 6 patients without an elevated cyclin D (none). The relative level of expression of each gene in each sample is shown. For cyclin D1 (CCND1), the expression of the long-form mRNA is in dark green and total mRNA is in light green. The fifth line shows a PI calculated from the log of the median of the normalized values for 12 genes associated with proliferation. The values for PB were extrapolated from a HuFL dataset.16 The bottom line shows a chromosome 3, 5, 7, 9, 11, 15, 19, 21 index (CI) calculated from the median of the normalized values for genes on a given chromosome. The deviation from 1 for the average of 8 chromosomes (3, 5, 7, 9, 11, 15, 19, and 21) is plotted.
