MLL WT expression is induced in primary MLL^PTD/WT blasts treated with 5′-Aza-CdR and depsipeptide. Primary blasts were incubated with media alone or with 5′-Aza-CdR and depsipeptide, singly or in combination, as described in “Materials and methods.” RNA was extracted from viable cells and cDNA was prepared for QRT-PCR assays. MLL PTD and MLL WT transcript levels were determined, and the results are presented as fold increase in MLL WT/MLL PTD transcript ratio relative to the media control sample for each patient AML sample. No MLL WT/MLL PTD ratio is depicted in the graph for media-only controls since no MLL WT was detected under this condition. For those samples in which a treatment condition did not induce MLL WT, the ratio is set to zero, although MLL PTD transcript is present, and these results are represented by dots on the x-axis. Horizontal bars represent mean values.