Figure 1.
![Figure 1. Generation of L-selectin-deficient lymphoma cells. (A) Genotype of T-lymphoma cell lines generated from normal (164T2 and 267), heterozygous (1681), and L-selectin-deficient mice (KOL lines) showing the presence of the wild-type allele (728 base pair [bp]) and the puromycin-containing mutant alleles (320 bp). (B) Surface expression of L-selectin on lymphoma cell lines. Cells were stained for L-selectin using the MEL-14 mAb by flow cytometry. Controls included cells incubated with SA-PE alone. The T-cell origin of all lymphoma cell lines was established by their ability to express CD3ϵ (data not shown). The x axis measures fluorescence intensity; the y axis, cell counts.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/12/10.1182_blood-2004-04-1406/5/zh80120580040001.jpeg?Expires=1724254751&Signature=V3esgn5BsmTVzCL4zxAc5v9NW9i0duj7i4vLl3Q2qTpAJGCm3422Yd~J4GPVaoFyZzareIDSTGaUe3Zxay1X54QJUMUC1mBM~9hs4h-SGeQ595qPE84kIIjn9F4FK-ZDZe~KZ9qgtekG6CH8ztv-2SOhblXNTSh8tvQC92YLU2j1zKwcN7MGUqnnnDhgHOE3Nyi~Jsb63QzFnaBjPl8F0qbTORMB5AiAHxPg4bnVjVw8k~9xqCoDRzATsGzF3-gECaSOH6Z4i886-XL2SoG0LqJnwK9DdP9~2Wuiav1uHVRAOv5pMKHtjTBquE1Oz747YeYdAmw1M7L-dwaWQvdUrw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Generation of L-selectin-deficient lymphoma cells. (A) Genotype of T-lymphoma cell lines generated from normal (164T2 and 267), heterozygous (1681), and L-selectin-deficient mice (KOL lines) showing the presence of the wild-type allele (728 base pair [bp]) and the puromycin-containing mutant alleles (320 bp). (B) Surface expression of L-selectin on lymphoma cell lines. Cells were stained for L-selectin using the MEL-14 mAb by flow cytometry. Controls included cells incubated with SA-PE alone. The T-cell origin of all lymphoma cell lines was established by their ability to express CD3ϵ (data not shown). The x axis measures fluorescence intensity; the y axis, cell counts.
