Generation of NUP98-HOXD13 (NHD13) transgenic mice. (A) Schematic of NHD13 fusion. The fusion point, NUP98 GLFG repeats, and HOXD13 homeodomain are indicated. (B) Construct used for pronuclear injection. The 5′ and 3′ vav regulatory elements, simian virus 40 (SV40) intron and polyadenylation signal, and NHD13 fusion cDNA are indicated. (C) Northern blot analysis of NHD13 expression. (Top) Human NUP98 probe and (bottom) L32 probe (used as an RNA-loading control). Br indicates brain; Th, thymus; Li, liver; Sp, spleen; Ki, kidney; BM, bone marrow. (D) Western blot of lysates from control and NHD13 transgenic thymocytes. A 53 kDa band is seen only in the transgenic thymocytes. The blot was reprobed with tubulin as a loading control.