Survival and proliferation of DN cells are impaired in wt LNs. (A) Analysis of cell cycle status of DN phenotype cells. Forty minutes after injection of 1 mg BrdU intraperitoneally, mice were killed and cells were stained with 7AAD and antibodies against BrdU, CD25, CD44, and lineage markers. Numbers correspond to the percentages of cells in the G_1/0, S, and G₂+M phase of the cell cycle. One representative experiment out of 3. (B) Lin^– CD44⁺c-Kit subsets in cycling thymocytes. Mice received 2 injections (1 mg each) of BrdU at 2-hour intervals. Twenty-four hours later, prepared cells were stained with antibodies against BrdU, CD44, c-Kit, and lineage markers (which included CD25). Numbers indicate the percentage of BrdU⁺ cells. One representative experiment out of 3. (C) Percentages of annexin V⁺ cells in wt thymus, wt LNs, and OM⁺ LNs are indicated in the graphs.