Immunophenotype of malignant cells from recipients of FLT3-TKD mutants. (A) Flow cytometric analysis of bone marrow and thymus cells from mice receiving 30 000 FLT3 D835Y-positive bone marrow cells (FLT3 D835Y 30^high). Flow cytometric analysis of (B) bone marrow (BM), peripheral blood (PB), thymus, lymph node (LN) cells, and (C) bone marrow, peripheral blood, and lymph node cells from mice receiving 318 000 FLT3 D835Y-positive bone marrow cells (FLT3 D835Y 318^high). (B) The T-cell phenotype. (C) The B-ALL phenotype. (D) Flow cytometric analysis of bone marrow, peripheral blood, and thymus cells from mice receiving 240 000 FLT3 I836M+R-positive bone marrow cells (FLT3 I836M+R 240^high). Two-parameter dot plots show expression of lineage-specific antigens (CD11b, B-220, Thy1.2, CD4, CD8) versus EGFP. Numbers indicate the percentage of cells in each quadrant.