GVHD T cells cause colitis when transferred into syngeneic Rag mice. Lethally irradiated (900 cGy) Balb mice received transplants of TCD B6 BM plus 3 × 105 B6 spleen cells. Mice were killed 20 to 27 days after transplantation. Spleen cell suspensions were then transferred into either B6 (▪, n = 18) or Balb (□, n = 17) Rag mice. In most experiments, spleen cells were pooled from multiple GVHD animals before transfer into Rag recipients. Mice in both cohorts received an equivalent number of T cells in the spleen cell suspension, ranging between 0.3 and 1 × 106 in replicate experiments. Survival (A), serial weight curves (B), spleen cellularity (C), and total number of splenic T cells (D) are shown. Data in panels C and D are presented as the mean ± SEM. (E) Incidence of colitis in B6 and Balb Rag recipients of GVHD T cells. Data are derived from 6 independent experiments.
