Impaired migration of p85α^–/– BMMs in a wound-healing assay. (A) WT and p85α^–/– BMMs were cultured for 8 days in 24-well plates in IMDM/20% FBS/100 ng/mL M-CSF. An artificial wound was created in the macrophage monolayer using a pipette tip. Photographs were taken immediately and again at indicated time periods after creating the wound. Data are from one representative experiment. Similar results were obtained in 2 independent experiments. Images were acquired through an Olympus TH3 microscope equipped with a 20×/0.4 objective lens (Olympus, Melville, NY), and were captured with a Nikon HRD100 camera (Nikon, Melville, NY). (B) Quantification of the wound-healing assay. The figure shows the mean number of cells migrated ± SD. Data are from one representative experiment. *P<.05 for WT versus p85α^–/–.